Effects of Hydroalcoholic Extract of Matricaria Recutita L. on Lipid Peroxidation and Nitric Oxide in Pentylenetetrazole-kindled Mice Ameliorative effects of hydroalcoholic extract of Matricaria recutita L. on epilepsy
Iranian Journal of Pharmaceutical Sciences,
Vol. 17 No. 2 (2021),
1 April 2021
,
Page 37-50
https://doi.org/10.22037/ijps.v17.40285
Abstract
Matricaria recutita L. (chamomile) is a well-known medicinal plant. As the main constituents of chamomile, flavonoids such as apigenin, act on benzodiazepine/GABA receptors. This study was designed to determine the protective effect of hydroalcoholic Matricaria recutita (MR) extract (hMRxt) on pentylenetetrazole (PTZ)-induced kindling model and lipid peroxidation in mice. Forty-eight male albino mice (25-30 g) were randomly divided into six groups (n=8). Kindling was induced by 13 PTZ injections (35 mg /kg; i.p.) every other day for 26 days. The seizure score was observed and noted until 30 minutes after the PTZ injection. Finally, the mice were decapitated and their brains were dissected out so as to assess some biochemical factors, namely malondialdehyde (MDA) and nitric oxide metabolites (NOx) in the brain tissue. One-way ANOVA was used for analysis in Prism 6.0. The results showed that hMRxt (400 mg/kg) and valproate (VAP) (150 mg/kg) significantly decreased the progression and duration of seizure induced by PTZ (P <0.001). NOx level in the hMRxt (400 mg / kg), VAP (150 mg / kg) and the combination of hMRxt (50 mg / kg) + VAP (50 mg / kg) groups was significantly reduced compared to the PTZ group. Also, hMRxt (50 and 400 mg/kg) significantly increased the MDA level (P <0.001) compared to PTZ and control groups. This study revealed that hMRxt protects mice against the PTZ-induced epilepsy via NO signaling with no effect on lipid peroxidation.
Keywords:
- Epilepsy
- Pentylenetetrazol
- Kindling
- Malondialdehyde
- Nitric oxide
- Mice
How to Cite
Kazemi, M. ., Ardjmand, A. ., Esmaeil Shahaboddin, M. ., Mehrand, M. ., Banitaba-Bidgolia, S. M. ., & Ghavipanjeh, G. . (2021). Effects of Hydroalcoholic Extract of Matricaria Recutita L. on Lipid Peroxidation and Nitric Oxide in Pentylenetetrazole-kindled Mice: Ameliorative effects of hydroalcoholic extract of Matricaria recutita L. on epilepsy. Iranian Journal of Pharmaceutical Sciences, 17(2), 37–50. https://doi.org/10.22037/ijps.v17.40285
References
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[15] Bhaskaran N, Shukla S, Srivastava JK, Gupta S. Chamomile: an anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity. Int. J .Mol .Med. (2010) 26(6): 935-940.
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[23] Sebai H, Jabri M-A, Souli A, Rtibi K, Selmi S, Tebourbi O, ElBenna J, Sakly M. Antidiarrheal and antioxidant activities of chamomile (Matricaria recutita L.) decoction extract in rats. J. Ethnopharmacol. (2014) 152(2): 327-332.
[24] Rahmati B, Khalili M, Roghani M, Ahghari P. Anti-epileptogenic and antioxidant effect of Lavandula officinalis aerial part extract against pentylenetetrazol-induced kindling in male mice. J. Ethnopharmacol. (2013) 148(1): 152-157.
[25] Homayoun H, Khavandgar S, Dehpour AR. The role of α2‐adrenoceptors in the modulatory effects of morphine on seizure susceptibility in mice. Epilepsia (2002) 43(8): 797-804.
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[28] Sun J, Zhang X, Broderick M, Fein H. Measurement of nitric oxide production in biological systems by using Griess reaction assay. Sensors (2003) 3(8): 276-284.
[29] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. (1976) 72(1): 248-254.
[30] Wasowski C, Marder M. Flavonoids as GABAA receptor ligands: the whole story? J. Exp. Pharmacol. (2012) 4: 9.
[31] Jahani R, Mojab F, Mahboubi A, Nasiri A, Tahamtani A, Faizi M. An In-vivo study on anticonvulsant, anxiolytic, and sedative-hypnotic effects of the polyphenol-rich Thymus kotschyanus extract; evidence for the involvement of GABA-A Receptors. Iran J. Pharm. Res. (2019) 18(3):1456-1465.
[32] Jahani R, Khaledyan D, Jahani A, Jamshidi E, Kamalinejad M, Khoramjouy M, Faizi M. Evaluation and comparison of the antidepressant-like activity of Artemisia dracunculus and Stachys lavandulifolia ethanolic extracts: an in vivo study. Res. Pharm. Sci. (2019) 4(6):544-553.
[33] Salehi B, Venditti A, Sharifi-Rad M, Kręgiel D, Sharifi-Rad J, Durazzo A, Lucarini M, Santini A, Souto EB, Novellino E. The therapeutic potential of apigenin. Int. J. Mol. Sci. (2019) 20(6): 1305.
[34] Han JY, Ahn SY, Kim CS, Yoo SK, Kim SK, Kim HC, Hong JT, Oh KW. Protection of apigenin against kainate-induced excitotoxicity by anti-oxidative effects. Biol.Pharm. Bull. (2012) 35(9): 1440-1446.
[35] Rana A, Musto AE. The role of inflammation in the development of epilepsy. J. Neuroinflammation (2018) 15(1): 144.
[36] Srivastava JK, Pandey M, Gupta S. Chamomile, a novel and selective COX-2 inhibitor with anti-inflammatory activity. Life Sci. (2009) 85(19-20): 663-669.
[37] Zargaran A, Borhani-Haghighi A, Faridi P, Daneshamouz S, Kordafshari G, Mohagheghzadeh A. Potential effect and mechanism of action of topical chamomile (Matricaria chammomila L.) oil on migraine headache: A medical hypothesis.Med.Hypotheses (2014) 83(5): 566-569.
[38] Grosso C, Valentão P, Ferreres F, B Andrade P. The use of flavonoids in central nervous system disorders. Curr. Med. Chem. (2013) 20(37): 4694-4719.
[39] Naziroglu M, Akay MB, Celik O, Yildirim MI, Balci E, Yurekli VA. Capparis ovata modulates brain oxidative toxicity and epileptic seizures in pentylentetrazol-induced epileptic rats. Neurochem. Res. (2013) 38(4): 780-788.
[40] Hoffman M. A new role for gases: neurotransmission. Science (1991) 252(5014): 1788.
[41] De la Torre J, Pappas B, Prevot V, Emmerling M, Mantione K, Fortin T, Watson M, Stefano G. Hippocampal nitric oxide upregulation precedes memory loss and A β 1-40 accumulation after chronic brain hypoperfusion in rats. Neurol. Res. (2003) 25(6): 635-641.
[42] Wojtal K, Gniatkowska-Nowakowska A, Czuczwar SJ. Is nitric oxide involved in the anticonvulsant action of antiepileptic drugs. Pol. J. Pharmacol. (2003) 55: 535-542.
[43] Itoh K, Watanabe M, Yoshikawa K, Kanaho Y, Berliner LJ, Fujii H. Magnetic resonance and biochemical studies during pentylenetetrazole-kindling development: the relationship between nitric oxide, neuronal nitric oxide synthase and seizures. Neuroscience (2004) 129(3): 757-766.
[44] Kozlov AV, Biagini G, Tomasi A, Zini I. Ex vivo demonstration of nitric oxide in the rat brain: effects of intrastriatal endothelin-1 injection. Neurosci. Lett. (1995) 196(1-2): 140-144.
[2] Ngugi AK, Kariuki S, Bottomley C, Kleinschmidt I, Sander J, Newton C. Incidence of epilepsy: a systematic review and meta-analysis. Neurology (2011) 77(10): 1005-1012.
[3] Stafstrom CE, Carmant L. Seizures and epilepsy: an overview for neuroscientists. Cold .Spring Harb .Perspect. Med. (2015) 5(6): a022426.
[4] Sefil F, Arık AE, Acar MD, Bostanci MÖ, Bagirici F, Kozan R. Interaction between carbenoxolone and valproic acid on pentylenetetrazole kindling model of epilepsy. Int. J. Clin. Exp. Med. (2015) 8(7): 10508.
[5] Zhu X, Shen K, Bai Y, Zhang A, Xia Z, Chao J, Yao H. NADPH oxidase activation is required for pentylenetetrazole kindling-induced hippocampal autophagy. Radic. Biol. Med. (2016) 94: 230-242.
[6] Choudhary N, Bijjem KR, Kalia AN. Antiepileptic potential of flavonoids fraction from the leaves of Anisomeles malabarica. J. Ethnopharmacol. (2011) 135(2): 238-242.
[7] Kiasalari Z, Khalili M, Roghani M, Sadeghian A. Antiepileptic and Antioxidant Effect of Brassica nigra on Pentylenetetrazol-Induced Kindling in Mice. Iran J. Pharm. Res. (2012) 11(4): 1209-1217.
[8] Aguiar CC, Almeida AB, Araujo PV, de Abreu RN, Chaves EM, do Vale OC, Macedo DS, Woods DJ, Fonteles MM, Vasconcelos SM. Oxidative stress and epilepsy: literature review. Oxid. Med. Cell. Longev. (2012) 2012: 795259.
[9] Malinska D, Kulawiak B, Kudin AP, Kovacs R, Huchzermeyer C, Kann O, Szewczyk A, Kunz WS. Complex III-dependent superoxide production of brain mitochondria contributes to seizure-related ROS formation. Biochim. Biophys. Acta. (2010) 1797(6): 1163-1170.
[10] Ardjmand A, Shahaboddin ME, Mazoochi T, Ghavipanjeh G. Ameliorative effects of cerebrolysin against isoproterenol-induced myocardial injury in male rats. Life Sci. (2019) 227: 187-192.
[11] Menon B, Ramalingam K, Kumar RV. Oxidative stress in patients with epilepsy is independent of antiepileptic drugs. Seizure (2012) 21(10): 780-784.
[12] Shin EJ, Jeong JH, Chung YH, Kim WK, Ko KH, Bach JH, Hong JS, Yoneda Y, Kim HC. Role of oxidative stress in epileptic seizures. Neurochem. Int. (2011) 59(2): 122-137.
[13] Banach M, Piskorska B, Czuczwar SJ, Borowicz KK. Nitric oxide, epileptic seizures, and action of antiepileptic drugs. CNS. Neurol. Disord.Drug Targets (2011) 10(7): 808-819.
[14] Can ÖD, Özkay ÜD, Kıyan HT, Demirci B. Psychopharmacological profile of Chamomile (Matricaria recutita L.) essential oil in mice. Phytomedicine (2012) 19(3-4): 306-310.
[15] Bhaskaran N, Shukla S, Srivastava JK, Gupta S. Chamomile: an anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity. Int. J .Mol .Med. (2010) 26(6): 935-940.
[16] Kheiripour N, Karimi J, Khodadadi I, Tavilani H, Goodarzi MT, Hashemnia M. Silymarin prevents lipid accumulation in the liver of rats with type 2 diabetes via sirtuin1 and SREBP-1c. J. Basic. Clin. Physiol. Pharmacol. (2018) 29 (3): 301-308.
[17] Brodie MJ. Antiepileptic drug therapy the story so far. Seizure (2010) 19 (10): 650-655.
[18] Srivastava JK, Shankar E, Gupta S. Chamomile: A herbal medicine of the past with a bright future. Mol. Med. Rep. (2010) 85(19-20): 663-669.
[19] Keefe JR, Mao JJ, Soeller I, Li QS, Amsterdam JD. Short-term open-label chamomile (Matricaria chamomilla L.) therapy of moderate to severe generalized anxiety disorder. Phytomedicine (2016) 23(14): 1699-1705.
[20] Abbas B, Ahmad J, Hasan A, Mohsen T, Abolfazl A, Hosein A. Effects of the alcoholic extract of white mulberry leaves on behavioral performance of rats.Biomed .Pharmacol.J. (2015) 8715-719.
[21] Mobasher M, Sasani P, Aledavood SJ, Aramesh K, Larijani B. Revision of the guideline for ethical use of animals. J. Med. Ethics Hist. Med. (2012) 5(1): 70-111.
[22] Shoorei H, Khaki A, Ainehchi N, Taheri MMH, Tahmasebi M, Seyedghiasi G, Ghoreishi Z, Shokoohi M, Khaki AA, Raza SH. Effects of Matricaria chamomilla extract on growth and maturation of isolated mouse ovarian follicles in a three-dimensional culture system. Chin. Med. J. (2018) 131(2): 218.
[23] Sebai H, Jabri M-A, Souli A, Rtibi K, Selmi S, Tebourbi O, ElBenna J, Sakly M. Antidiarrheal and antioxidant activities of chamomile (Matricaria recutita L.) decoction extract in rats. J. Ethnopharmacol. (2014) 152(2): 327-332.
[24] Rahmati B, Khalili M, Roghani M, Ahghari P. Anti-epileptogenic and antioxidant effect of Lavandula officinalis aerial part extract against pentylenetetrazol-induced kindling in male mice. J. Ethnopharmacol. (2013) 148(1): 152-157.
[25] Homayoun H, Khavandgar S, Dehpour AR. The role of α2‐adrenoceptors in the modulatory effects of morphine on seizure susceptibility in mice. Epilepsia (2002) 43(8): 797-804.
[26] Ilhan A, Gurel A, Armutcu F, Kamisli S, Iraz M. Antiepileptogenic and antioxidant effects of Nigella sativa oil against pentylenetetrazol-induced kindling in mice. Neuropharmacology (2005) 49(4): 456-464.
[27] Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. (1978) 52: 302-310.
[28] Sun J, Zhang X, Broderick M, Fein H. Measurement of nitric oxide production in biological systems by using Griess reaction assay. Sensors (2003) 3(8): 276-284.
[29] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. (1976) 72(1): 248-254.
[30] Wasowski C, Marder M. Flavonoids as GABAA receptor ligands: the whole story? J. Exp. Pharmacol. (2012) 4: 9.
[31] Jahani R, Mojab F, Mahboubi A, Nasiri A, Tahamtani A, Faizi M. An In-vivo study on anticonvulsant, anxiolytic, and sedative-hypnotic effects of the polyphenol-rich Thymus kotschyanus extract; evidence for the involvement of GABA-A Receptors. Iran J. Pharm. Res. (2019) 18(3):1456-1465.
[32] Jahani R, Khaledyan D, Jahani A, Jamshidi E, Kamalinejad M, Khoramjouy M, Faizi M. Evaluation and comparison of the antidepressant-like activity of Artemisia dracunculus and Stachys lavandulifolia ethanolic extracts: an in vivo study. Res. Pharm. Sci. (2019) 4(6):544-553.
[33] Salehi B, Venditti A, Sharifi-Rad M, Kręgiel D, Sharifi-Rad J, Durazzo A, Lucarini M, Santini A, Souto EB, Novellino E. The therapeutic potential of apigenin. Int. J. Mol. Sci. (2019) 20(6): 1305.
[34] Han JY, Ahn SY, Kim CS, Yoo SK, Kim SK, Kim HC, Hong JT, Oh KW. Protection of apigenin against kainate-induced excitotoxicity by anti-oxidative effects. Biol.Pharm. Bull. (2012) 35(9): 1440-1446.
[35] Rana A, Musto AE. The role of inflammation in the development of epilepsy. J. Neuroinflammation (2018) 15(1): 144.
[36] Srivastava JK, Pandey M, Gupta S. Chamomile, a novel and selective COX-2 inhibitor with anti-inflammatory activity. Life Sci. (2009) 85(19-20): 663-669.
[37] Zargaran A, Borhani-Haghighi A, Faridi P, Daneshamouz S, Kordafshari G, Mohagheghzadeh A. Potential effect and mechanism of action of topical chamomile (Matricaria chammomila L.) oil on migraine headache: A medical hypothesis.Med.Hypotheses (2014) 83(5): 566-569.
[38] Grosso C, Valentão P, Ferreres F, B Andrade P. The use of flavonoids in central nervous system disorders. Curr. Med. Chem. (2013) 20(37): 4694-4719.
[39] Naziroglu M, Akay MB, Celik O, Yildirim MI, Balci E, Yurekli VA. Capparis ovata modulates brain oxidative toxicity and epileptic seizures in pentylentetrazol-induced epileptic rats. Neurochem. Res. (2013) 38(4): 780-788.
[40] Hoffman M. A new role for gases: neurotransmission. Science (1991) 252(5014): 1788.
[41] De la Torre J, Pappas B, Prevot V, Emmerling M, Mantione K, Fortin T, Watson M, Stefano G. Hippocampal nitric oxide upregulation precedes memory loss and A β 1-40 accumulation after chronic brain hypoperfusion in rats. Neurol. Res. (2003) 25(6): 635-641.
[42] Wojtal K, Gniatkowska-Nowakowska A, Czuczwar SJ. Is nitric oxide involved in the anticonvulsant action of antiepileptic drugs. Pol. J. Pharmacol. (2003) 55: 535-542.
[43] Itoh K, Watanabe M, Yoshikawa K, Kanaho Y, Berliner LJ, Fujii H. Magnetic resonance and biochemical studies during pentylenetetrazole-kindling development: the relationship between nitric oxide, neuronal nitric oxide synthase and seizures. Neuroscience (2004) 129(3): 757-766.
[44] Kozlov AV, Biagini G, Tomasi A, Zini I. Ex vivo demonstration of nitric oxide in the rat brain: effects of intrastriatal endothelin-1 injection. Neurosci. Lett. (1995) 196(1-2): 140-144.
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