Effects of Cells Density and Positioning on Optimized FTIR Biospectroscopy Effect of cell density on FTIR spectra
Iranian Journal of Pharmaceutical Sciences,
Vol. 10 No. 2 (2014),
1 April 2014
,
Page 61-68
https://doi.org/10.22037/ijps.v10.40827
Abstract
Researchers have found a big interest in biological application of Fourier Transform Infrared (FTIR) spectroscopy. Evaluating many diseases, staging them and studying the chemical structures of different formed compounds in diseases are some of the research applications of FTIR. Cancer is also one of these diseases. Researchers are trying to set up FTIR methods to detect and diagnose cancer cells and follow up the treatment steps using FTIR. In this regard, cancer cells and tissues are under investigation. In order to study cancer cells in lab, it is important to find out the proper cell density on the disk, at the first step. In this regard, the effect of different densities and positioning of cancer cells on FTIR supporting disk are studied in the present project. At the first step calibration of the instrument is checked using bovine serum albumin (BSA). Cancer cells were collected from culture dishes and washed with normal saline, twice. Different concentrations of cells (10000-320000 cells/uL) were located on ZnSe disks and dried prior to spectroscopy. The samples were scanned in the mid-infrared range of 4000-400 cm-1, with the resolution of 2 cm-1. Each spectrum was collected by 100 sample scans. Microscopic images of the disk were also taken to find out the distribution of the cells on the disk. The results of this study showed that the right amount of cell number and positioning on the disk is a very important parameter in bio-spectroscopic quality for biological purposes.
- A549
- A2780
- Cell
- Density
- FTIR
- HepG2
- Spectroscopy
- Spectrum
How to Cite
References
[2] Eri GK, Naik MC, Padma Y, Ramana MV, Madhu M and Gopinath C. Novel FT-IR spectroscopic method for the quantitation of atenolol in bulk and tablet formulations. JGTPS (2014) 5: 1750–1755.
[3] Andrus PGL and Strickland RD. Cancer grading by fourier transform infrared spectroscopy. Biospectroscopy (1998) 4: 37-46.
[4] Boskey AL and Mendelsohn R. Infrared spectroscopic characterization of mineralized tissues. Vib Spectrosc (2005) 38: 107–114.
[5] LeVine SM and Wetzel DL. Chemical analysis of multiple sclerosis lesions by FTIR microspectroscopy. Free RadicBiol Med (1998) 25: 33–41.
[6] Bakker Schut TC, Witjes MJH, Sterenborg HJCM, Speelman OC, Roodenburg JLN, Marple ET, Bruining HA and Puppels GJ. In vivo detection of dysplastic tissue by Raman spectroscopy. Anal Chem (2000) 72: 6010–6018.
[7] Conti C, Giorgini E, Pieramici T, Rubini C and Tosi G. FT-IR microscopy imaging on oral cavity tumors. J Mol Struct (2005) 187–193.
[8] Lasch P and Naumann D. FT-IR microspectroscopic imaging of human carcinoma thin sections based on pattern recognition techniques. Cell Mol Biol (1998) 44: 189–202.
[9] Stone N, Kendall C, Shepherd N, Crow P and Barr H. Near-infrared Raman spectroscopy for the classification of epithelial pre-cancers and cancers. J Raman Spectrosc. (2002) 33: 564–573.
[10] Bonnier F, Rubin S, Ventéo L, Krishna,CM, Pluot M, Baehrel B, Manfait M and Sockalingum GD. In-vitro analysis of normal and aneurismal human ascending aortic tissues using FT-IR microspectroscopy. Biochim Biophys Acta (2006) 1758: 968–973.
[11] Smith J, Kendall C, Sammon A, Christie-Brown J, Stone N. Raman spectral mapping in the assessment of axillary lymph nodes in breast cancer. Technol Cancer Res Treat (2003) 2: 327–332.
[12] Mourant JR, Yamada YR, Carpenter S, Dominique LR, Freyer JP. FTIR Spectroscopy Demonstrates Biochemical Differences in Mammalian Cell Cultures at Different Growth Stages. Biophys J (2003) 85: 1938–1947.
[13] Holman H-YN, Martin MC and Mckinney WR. Tracking chemical changes in a live cell: biomedical applications of SR- FTIR spectromicroscopy. Spectrosc-Int J (2003) 17: 139-159.
[14] Krafft C and Sergo V. Biomedical applications of Raman and infrared spectroscopy to diagnose tissues. Spectroscopy (2006) 20: 195–218.
[15] Protein secondary structural analysis by ftir. Shimadzu Analytical and Measuring Instruments, - - 2015. Disponivel em:
- Abstract Viewed: 241 times
- IJPS_Volume 10_Issue 2_Pages 61-68 Downloaded: 200 times