ArticleFTIR Determination of MiconazoleEffects on Mice Fetus Brain Tissue FTIR determination of Miconazole effects on Mice fetus brain tissue
Iranian Journal of Pharmaceutical Sciences,
Vol. 10 No. 2 (2014),
1 April 2014
,
Page 79-84
https://doi.org/10.22037/ijps.v10.40829
Abstract
Miconazole is an imidazole antifungal agent, commonly applied topically to the skin or mucous membranes. The aim of this study was to examine the alternative method for gaining mechanism or the bimolecular changes caused by the possible teratogenic effects of Miconazole on mice fetus brain tissueusing FTIR-Microspectroscopy.The mice were injected with Miconazole(60 mg/Kg) on gestation day 9. Fetuses were dissected on day 15 of gestation and morphological and histological studies on thefetus were carried out. Sections (10 μm) of controland Miconazoletreated fetus brain tissue were used for FTIR measurement in the mid-infraredregion. The results were shown by spectra2ndderivativeand also subtracting from control spectra.A lower intensity in the lipid (2800–3000 cm-1) and amid I (1600–1800 cm-1) regions of Miconazole treated mice fetus brain tissue was observed compared to the control mice fetus brain tissue. No major spectral shifting was observed at amide I band, amide II band and nucleic acid regions.As a conclusion, FTIR-Microspectroscopycan be a useful tool forteratogenic measurement with a unique ability to identify the modified bimolecular structuresin mice fetus tissues.
- Biospectroscopy
- FTIR-Microspectroscopy
- Mice fetus
- Miconazole
- Teratogenic
How to Cite
References
[2] Marc E, Manuelle DB, Jonathan H and Lieve G.Effectiveness of an otic product containing Miconazole, Polymyxin B and Prednisolone in the treatment of canine otitis externa: Multi site field trial in the US and Canada. Intern J Appl Res Vet Med. 2010, 8 (1): 21-30.
[3] Timothy J and et al. Fluconazole-Induced Congenital Anomalies in Three Infants. Clinical Infectious Diseases. 1996; 22:336-40.
[4] Tiboni GM, Marotta F, Castiglieo AP. Teratogenic effect in mouse fetuses subjected to in concurrent utero exposure to miconazole and metronidazole. Reproductive toxicology (2008) 26: 254-261.
[5] Edwards DI. Mechanisms of selective toxicity of metronidazole and other nitroimidazole drugs. Br J Vener Dis. (1980) 56: 285-90.
[6] Cantu JM, Garcia-Cruz D: Midline facial defect as a teratogenic effect of metronidazole. Birth defects (1982) 18: 85-88.
[7] Carvajal A, Sanchez A, Hurtarte G. Metronidazole during pregnancy (letter). Int J Gynecol Obstet (1995) 48: 323-324.
[8] Bendesky A, Menéndez D, Ostrosky-Wegman P. Is metronidazole carcinogenic? Mutat Res. (2002) 511(2):133-44.
[9] Falagas M, Walker A, Jick H, Ruthazer R, Griffith J and Snydman D. Late Incidence of Cancer After Metronidazole Use: A Matched MetronidazoleUser/Nonuser Study. Clinical Infectious Diseases (1998) 26:384–8.
[10] Metronidazole (2B). International Agency for Research on Cancer (IARC)- Summarizes and evaluations. (1987) 7: 250.
[11] Metronidazole- IARC monographs. (1987) 6: 394-398.
[12] Movasaghi Z, Rehman Sh, Ur Rehman I. Fourier Transform Infrared (FTIR) Spectroscopy of Biological Tissues. Applied Spectroscopy Reviews (2008) 43: 134–179.
[13] Ying-Jen Chen, Yih-DiCh, Hsin-Yi L, Paul-Yann L, Chia-Siu W. Observation of biochemical imaging changes in human pancreatic cancer tissue using Fourier- transform Infrared Microspectroscopy. Chang Gung Med (2006) 29: 518-27.
[14] Nakamura T, Kelly J, Trevisan J, Cooper L, Bentley A, Carmichael P, Scott A et al. Microspectroscopy of spectral biomarkers associated with human corneal stem cells. Molecular Vision (2010) 16:359-368.
[15] Mehrotra R, Gupta A, Kaushik A, Prakash N, Kandpal H. Indian J of Experimental Biology (2007) 45: 71-76.
[16] Fabian H, Lasch P, Boese M, Haensch W. Infrared microspectroscopic imaging of benign breast tumor tissue sections. Journal of Molecular Structure (2003) 661-662: 411–417.
[17] Mordechai S, sahu R. K, hammody Z, mark S, kantarovich k, Guterman H, Podshyvalov A, Goldstein J, Argov S. Possible common biomarkers from ftir microspectroscopy of cervical cancer and melanoma. Journal of microscopy (2004) 215: 86–91.
[18] Khalil S, Khodeir M, Hakam R, El-Monem Rezq R. Spectroscopic Study for Detection and Grading of Breast Carcinoma In vitro. Aus tralian Journal of Basic and Applied Sciences (2009) 3(3): 2419-2428.
[19] Ashtarinezhad et al. FTIR Microspectroscopy Reveals Chemical Changes in Mice Fetus Following Phenobarbital Administration. Iranian Journal of Pharmaceutical Research (2015),14 (Supplement): 121-130.
[20] Ashtarinezhad et al. FTIR-Microspectroscopy Detection of Metronidazole Teratogenic Effects on Mice Fetus. Iranian Journal of Pharmaceutical Research (2014), 13 (supplement): 105-115.
[21] The Guide for the Care and Use of Laboratory Animals published by the National Academy Press, which was accepted by the ethnic committee of the AUSR in Iran Washington, DC. 1996.
[22] Godwin A. Histochemical Uses of Haematoxylin - A Review. JPCS (2011) 1:24-34.
[23] S. Nafisi, A. Panahyab, G.B. Sadeghi. Interactions between β-carboline alkaloids and bovine serum albumin: investigation by spectroscopic approach. Journal of Luminescence 132 (2012) 2361–2366.
[24] Marotta F, Tiboni GM. Molecular aspects of azoles-induced teratogenesis. Expert Opin. Drug Metab. Toxicol. (2010) 6(4):461-482.
[25] Giavini E, Menegola E. Are azole fungicides a teratogenic risk for human conceptus. Toxicology Letters 198 (2010) 106–111.
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