Evaluation of Antinociceptive Activity of Berberis Vulgaris L. Fruit’s Hydroethanolic Extract in Male Mice Antinociceptive activity of Berberis vulgaris in male mice
Iranian Journal of Pharmaceutical Sciences,
Vol. 9 No. 4 (2013),
1 October 2013
,
Page 13-20
https://doi.org/10.22037/ijps.v9.40855
Abstract
Herbal medicine has an old history with a broad application all over the world. Many researches have focused on the curative as well as antinociceptive effects of herbal extract. The aim of this study was to investigate the analgesic effects of Berberis vulgaris hydroethanolic extract (BVE) in male mice. 30 male mice were divided into 5 groups: control, treated by morphine, treated by BVE (150 & 300 mg/kg) and naloxane plus BVE (300 mg/kg) randomly. To assess the antinociceptive effects of BVE, the animals were examined by employing different pain models such as, tail-flick tests (for acute pain) and acetic acid-induced writhing (for chronic pain) after treatment with morphine, naloxane and BVE. The results indicate that the BVE showed an important antinociceptive effect at doses of 150 and 300 mg/kg (P<0.01 and P<0.001, respectively), administered intraperitoneally in mice, which significantly inhibited the abdominal constrictions (55.73% of inhibition) induced by acetic acid and increase tail-flick latency when compared to control group in the same dose, although they were less effective than morphine in the same assay. The antinociceptive models employed here reveal a potential analgesic effect of the Berberis vulgaris hydroethanolic extract. We suggest that this effect might be caused by anti-inflammatory effect and the stimulation of the opioid receptors.
- Berberis vulgaris
- analgesia
- tail flick
- morphine
- naloxone
- mice
How to Cite
References
[2] Dale M, Rang H, Dale M. Rang & Dale’s pharmacology. [Edinburgh]: Churchill Livingstone (2007).
[3] Imanshahidi M, Hosseinzadeh H.Pharmacological and therapeutical effects of Berberis vulgaris and its active constituent, berberine. Phytother Res (2008)22: 999-1012.
[4] Ivanovska N, Philipov S. Study on the antiinflamatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. Int J Immunopharmacol (1996)18: 553-561.
[5] Kupeli E, Kosar M, Yesilada E, Husnu K, Baser C. A comparative study on the anti-inflamatory, antinociceptive and antipyretic effects of isoquinoline alkaloids from the root of Turkish Berberis species. Life Sci (2002)72: 645-657.
[6] Freile ML, Giannini F, Pucci G. Antimicrobial activity of aqueolus extracts of berberine isolates from Berberis heterophylla. Fitoterapia (2003)74: 702-705.
[7] Mahady GB, Pendland SL, Stoia A, Chaadwick, LR. In vitro susceptibility of Helicobacter pylorito isoquinoline alkaloids from Sanguinaria Canadensis and Hydrastis canadensis. Phytother Res (2003)17: 217-221.
[8] Iizuka N, Miyamoto K, Okita K, Tangoku A, Hayashi H, Yosino S. Inhibitory effect of Coptidis rhizoma and berberine on the proliferation of human esophageal cancer cell lines. Cancer Lett (2000)148: 19-25.
[9] Thirupurasundari CJ, Padmini R, Devaraj SN.Effect of berberine on the antioxidant status, ultrastructural modifications and protein bound carbohydrates in azoxymethane-induced colon cancer in rats. Chem Biol Interact (2009)177:190-195.
[10]Wang N, Feng Y, Zhu M, Tsang CM, Man K, Tong Y, Tsao SW. Berberine induces autophagic cell death and mitochondrial apoptosis in liver cancer cells: The cellular mechanism. J Cell Biochem (2010)111: 1426-
1436.
[11] Kuo CL, Chi CW, Liu TY. The anti-inflamatory potential of berberine in vitro and in vivo. Cancer Lett (2004)203: 127-137.
[12] Singh A, Duggal S, Kaur N, Singh J. Berberine:Alkaloid with wide spectrum of pharmacological activities. J Nat Prod (2010)3:64-75.
[13] Saied S, Begum S. Phytochemical studies of Berberis vulgaris. Chem Nat Compd (2004)40:137-140 .
[14] Fatehi M, Saleh TM, Fatehi-Hassanabad Z, Farrokhfal K, Jafarzadeh M, Davodi SA. Pharmacological study on Berberis vulgaris fruit extract. J Ethnopharmacol (2005)102: 46-52.
[15] Lee B, Yang CH, Hahm DH, Choe ES, Lee HJ, Pyun KH, Shim I. Inhibitory Effects of Coptidis rhizome and Berberine on Cocaine-induced Sensitization. Evid Complement Altern Med (2009)6: 85-90.
[16] D’Amour FE, Smith DL. A method of determining loss of pain sensation. J Pharmacol and Experim Therap (1941)27: 74-79.
[17] Collier HO, Dinneen LC, Johnson CA, Schneider C. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol (1968)32: 295-310.
[18] Reanmongkol W, Matsumoto K, Watanabe H, Subhadhirasakul S, Sakai SI. Antinociceptive and antipyretic effects of alkaloids extracted from the stem bark of Hunteria zeylanica. Biol Pharm Bull (1994)17: 1345-1350.
[19] Ghannadi A, Hajhashemi V, Jafarabadi H. An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols. J Med Food (2005)8: 488-493.
[20] Hajhashemi V, Ghannadi A, Sharif B. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia Mill. J Ethnopharmacol (2003)89: 67-71.
[21] Hajhashemi V, Sajjadi SE, Heshmati M. Anti-inflammatory and analgesic properties of Heracleum persicum essential oil and hydroalcoholic extract in animal models. J Ethnopharmacol (2009)124: 475-480.
[22] Hajhashemi V, Sajjadi SE, Zomorodkia M. Antinociceptive and antiinflammatory activities of Bunium persicum essential oil, hydroalcoholic and polyphenolic extracts in animal models. Pharm Biol (2011)49: 146-151.
[23] Bors W, Saran M. Radical scavenging by flavonoid antioxidants. Free Radic Res Commun (1987)2: 289-294.
[24] Amresh G, Reddy GD, Rao C, Singh PN. Evaluation of anti-inflammatory activity of Cissampelos pareira root in rats. J Ethnopharmacol (2007)110: 526-531.
- Abstract Viewed: 248 times
- IJPS_Volume 9_Issue 4_Pages 13-20 Downloaded: 222 times