The Effect of Beta-Cyclodextrin on the Iodometric Determination of Some Penicillins Reported in USP and BP Iodometric determination of penicillins
Iranian Journal of Pharmaceutical Sciences,
Vol. 8 No. 4 (2012),
1 October 2012
,
Page 227-231
https://doi.org/10.22037/ijps.v8.40921
Abstract
This work summarizes how the efficiency of pharmacopeias (USP and BP) procedures for the iodometric determination of pencillins can be significantly enhanced through the use of beta-cyclodextrin (β-CD). In the commonly used iodometric method, titration takes about 30 min; 15 min to hydrolyze the antibiotics and 15 min for reaction between iodine and hydrolyzed species. The experimental results indicate that β-CD accelerates both hydrolysis of amoxicillin, ampicilline and cloxacilline and the reaction of their hydrolyzed products with iodine. Effect of different parameters such as sodium hydroxide, hydrochloric acid concentrations, and the waiting times in the presence of β-CD on the total titration times reported in pharmacopeia are evaluated. Finally, it is shown that the analysis times of iodometric assay for ampiciline an d amoxicilin in the presence of 0.108 mg.ml-1 β-CD are reduced from 30 to 5 min and those of cloxaciline is reduce to 10 min. The iodometric titration in the presence of β-CD is referred to as modified USP. Student t-tests at 95% level shows that there are no significant differences between USP and Modified USP. It is also shown that β-CD increases the stability of penicillin-G relative to USP buffer No.1. The reliability of the proposed method is evaluated by analysis of the above mentioned antibiotics in different pharmaceutical forms. The results show that the modified method can be successfully applied for the assay of cloxaciline injection vial, amoxicillin and ampicillin capsules and suspensions.
- β-Cyclodextrin
- Iodometric assay
- Penicillins
- Pencilin G Stability
How to Cite
References
[2] The United States Pharmacopia, Newyork, (2007); 1(2000), 129-132; 2(2003), 1961-3.
[3] Pariot N, Edwards-Le´vy F, Andry MC, Le´vy MC. Cross-linked b-cyclodextrin microcapsules. II. Retarding effect on drug release through semipermeable membranes. Int J Pharm 2002; 232: 175-81.
[4] Lin SZ, Wouessidjewe D, Poelman MC, Duchene D. In vivo evaluation of indomethacin/cyclodextrin complexes gastrointestinal tolerance and anti-inflammatory activity. Int J Pharm 1994;106: 63-7.
[5] Fetzner A, Bohm S, Schreder S, Schubert R.Degradation of raw or film-incorporated ß cyclodextrin by enzymes and colonic bacteria. Eur J Pharm Biopharm 2004; 58: 91-7.
[6] Pose-Vilarnovo B, Perdomo-Lo´pez I, Echezarreta-Lo´pez M, Schroth-Pardo P, Estrada E. Torres-Labandeira JJ. Improvement of water solubility of sulfamethizole through its complexation with ß- and hydroxypropyl-ß bcyclodextrin: characterization of the interaction in solution and in solid state. Eur J Pharm Sci 2001; 13: 325-31.
[7] Boudad H, Lebas G, Cheron M, Ducheˆne D, Ponchel G. Combined hydroxypropyl- - cyclodextrin and poly(alkylcyanoacrylate) nanoparticles intended for oral administration of saquinavir. Int J Pharm 2001; 218: 113-24.
[8] Loftsson T, Masson M. Cyclodextrins in topical drug formulations: theory and practice. Int J Pharm 2001; 225: 15-30.
[9] Shanmugam M, Ramesh D, Nagalakshmi V, Kavitha R, Rajamohan R, Stalin T. Host-guest interaction of l-tyrosine with ß-cyclodextrin. Spectrochim Acta Part A 2008; 71: 125-32.
[10] Scondo A, Dumarcay F, Marsura A, Barth D.Tandem Staudinger-Aza-Wittig reaction in supercritical CO2: synthesis of a pharmaceutical interest compound. J Supercrit Fluids 2010; 53:60-3.
[11] Roth HJ, Eger K, Troschutz R. Pharmaceutical Chemistry. Ellis Horwood, 2, 1991, pp. 252-3.
[12] Dodziuk H. Cyclodextrin and their complexations: chemistry, analytical methods, applications. Wiley-VCH Verlag GmbH & Co., 2006, pp. 93-105.
[13] VanEtten RL, Sebastian JF, Clowes GA, Bender ML. Acceleration of phenyl ester cleavage by cycloamyloses. A model for enzyme specificity.J Am Chem Soc 1967; 89: 3242-53.
[14] Tutt DE, Schwartz MA. Specificity in the cyclohept-amylose-catalysed hydrolysis of penicillins. J Chem Soc Chem Commun 1970; 2:113-4.
[15] Schwartz MA, Tutt DE. Model catalysts which simulate penicillinase. V. The cyclohepta-amylosecatalyzed hydrolysis of penicillins. J Am Chem Soc 1971; 93: 767-72.
[16] Diaz D, Vargas-Baca I, Gracia-Mora J. Betacyclodextrin inclusion complexes with iodine: an advanced and inexpensive undergraduate chemistry experiment. J Chem Educ 1994; 71:708.
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