Bioresorbability Evaluation of Hydroxyapatite Nanopowdersin a Stimulated Body Fluid Medium Bioresorbability of hydroxyapatite nanopowders
Iranian Journal of Pharmaceutical Sciences,
Vol. 4 No. 2 (2008),
1 April 2008
,
Page 141-148
https://doi.org/10.22037/ijps.v4.41110
Abstract
The bone mineral consists of tiny hydroxyapatite (HA) crystals in the nanoregime.Nanostructured HAis also expected to have better bioactivity than coarser crystals.This paper reports on the in vitroevaluation of bone like HAnanopowders. The prepared HAnanopowder was characterized for its phase purity, chemical homogeneity and bioactivity. Fourier transform infrared (FT-IR) spectroscopy was used to identify the functional groups. X-ray diffraction analysis was carried out to study the phase composition. The in vitrotest was performed in a stimulated body fluid medium. The changes in the pH of SBF medium were measured at pre-determined time intervals using a pH meter. The dissolution of calcium ions in the SBF medium was determined by an atomic absorption spectrometer. FT-IR spectroscopy result combined with the X-ray diffraction exhibited single phase of HAwith carbonate peaks in the FT-IR spectrum. Results indicated that increasing the sintering temperature increases the crystallinity and the crystallite size of HA nanopowders. HAnanopowders ionic dissolution rate was found to be quite higher than conventional HAand closer to biological apatite owing to its nanostructure dimensions.
- Bioresorbability
- Hydroxyapatite
- Nanostructure.
How to Cite
References
[2]Currey J. Biomaterials: Sacrificial bonds heal bone. Nature2001; 414: 699-708.
[3]Aoki H. Medical applications of hydroxyapatite.Tokyo: Ishiyaku EuroAmerica, 1994.
[4]Murugan R, Ramakrishna S. Coupling of therapeutic molecules onto surface modified coralline hydroxyapatite. Biomaterials2004; 25:3073-80.
[5]Murugan R, Rao KP. Grafting of glycidyl methacrylate upon coralline hydroxyapatite in conjugation with demineralized bone matrix using redox initiating system. Macromolecules 2003; 11:14-8.
[6]Kivrak N, Tas AC. Synthesis of calcium hydroxyapatite tricalcium phosphate (HA-TCP)composite bioceramic powders and their sintering behavior. J Am Ceram Soc1998; 81: 2245-52.
[7]Driessens FCM, Boltong MG, De Maeyer EAP,Wenz R, Nies R, Planell JA. The Ca/Prange of nanoapatitic calcium phosphate cements.Biomaterials2002; 23: 4011-7.
[8]Kim HM. Ceramic bioactivity and related biomimetic strategy. Current Opinion Solid State Mater Sci2003; 7: 289-99.
[9]LeGeros RZ. Calcium phosphates in oral biology and medicine.Basel: Switzerland, Karger A G,1991; pp. 154-92.
[10]Radin SR, Ducheyne P. The effect of calcium phosphate ceramic composition and structure on in vitrobehavior, II. Precipitation. J Biomed Mater Res1993; 27: 35-45.
[11]Ducheyne P, Radin S, King L. The effect of calcium phosphate ceramic composition and structure on in vitrobehavior, I. Dissolution. J Biomed Mater Res1993; 27: 25-34.
[12]Harada Y, Wang JT, Doppalapudi VA, Willis AA,Jasty M, Harris WH, Nagase M, Goldring SR.Differential effects of different forms of hydroxyapatite and hydroxyapatite/tricalcium phosphate particulates on human monocyte/macrophages in vitro. J Biomed Mater Res1996;31: 19-26.
[13]Wise DL, Trantolo DJ, Lewandrowski KU,Gresser JD, Cattaneo MV, Yaszemski MJ.Orthopedic, dental and bone graft applications.Vol. 2. Totowa: Humana Press, 2000.
[14]Fathi MH, Hanifi A. Evaluation and characteri-zation of nanostructure hydroxyapatite powder prepared by sol-gel method.J Mater Lett2007;61: 3978-83.
[15]Jenkins R, Snyder RL. Introduction to X-ray powder diffractometry.New York: John Wiley &Sons, 1996; pp. 123-6.
[16]Landi E, Tampieri A, Celotti G, Sprio S.Densification behaviour and mechanisms of synthetic hydroxyapatites. J Eur Ceram Soc 2000;20: 2377-87.
[17]Ohtsuki C, Kokubo T, Yamamuro. Mechanism of apatite formation on CaO-SiO2-P2O5glasses in a simulated body fluid. J Non-Cryst Solids1992;143: 84-92.
[18]Varma HK, Babu SS. Synthesis of calcium phosphate bioceramics by citrate gel pyrolysis method. Ceram Int2005; 31: 109-14.
[19]Miyaji F, Kono Y, Suyama Y. Formation and structure of zinc-substituted calcium hydroxyapatite. Mater Res Bull 2005; 40: 209-20.
[20]Rehman I, Bonfield W. Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy. J Mater Sci Mater Med1997; 8: 1-4.
[21]Sinha A, Ingle A, Munim KR, Vaidya SN, Sharma BP, Bhisey AN. Development of calcium phosphate based bioceramics. Bull Mater Sci 2001; 24: 653-7.
[22]Chow LC, Sun L, Hockey B. Properties of nanostructured hydroxyapatite prepared by a spray drying technique. J Res Inst Stand Technol2004; 109: 543-51.
[23]Krajewski A, Mazzocchi M, Buldini PL,Ravaglioli A, Tinti A, Taddei P. Fagnano C.Synthesis of carbonated hydroxyapatite:Efficiency of the substitution and critical evaluation of analytical methods. J Mol Struct2005; 221:744-7.
[24]Emerson WH, Fischer EE. The infrared absorption spectra of carbonate in calcified tissues. Arch Oral Biol 1962; 7: 671-83.
[25]Elliott JC, Holcomb DW, Young RA. Infrared determination of the degree of substitution of hydroxyl by carbonate ions in human dental enamel. Calcif Tissue Int1985; 37: 372-5.
[26]Murugan R, Rao KP, Kumar TSS. Heat-deproteinated xenogeneic bone from slaughterhouse waste: Physico-chemical properties. Bull Mater Sci2003; 26: 523-8.
[27]Bouyer E, Gitzhofer F, Boulos MI. Morphological study of hydroxyapatite nanocrystal suspension.J Mater Sci Mater Med2000; 11: 523-31.
[28]Liu DM, Troczynski T, Tseng WJ. Water-basedsol-gel synthesis of hydroxyapatite: Process development. Biomaterials2001; 22: 1721-30.
[29]Kim HM, Kim Y, Park SJ, Rey C, Lee HM,Gimcher MJ, Ko JS. Thin film of low-crystalline calcium phosphate apatite formed at low temperature. Biomaterials2000; 21: 1129-34.
[30]Posner AS. Crystal chemistry of bone mineral.Physiol Rev1969; 49: 760-92.
- Abstract Viewed: 334 times
- IJPS_Volume 4_Issue 2_Pages 141-148 Downloaded: 211 times