Effect of Solvent Polarity on the Extraction Yield of Antioxidants from Lactobacillus Supernatants Effect of Solvent Polarity on the Antioxidant Extraction
Iranian Journal of Pharmaceutical Sciences,
Vol. 18 No. 1 (2022),
15 January 2022
,
Page 47-54
https://doi.org/10.22037/ijps.v18.41363
Abstract
There is increasing evidence to suggest that the Lactobacilli species possess antioxidant activities, however, there are a few reports of optimization of solvent systems for the separation of their antioxidant compounds by thin-layer chromatography. In the current study, we explore the efficiency of four organic solvents (aqueous, methanol, ethyl acetate, and n-hexane) for the extraction of antioxidant materials from Lactobacillus supernatants. According to the results, methanol extraction significantly increased the antioxidant properties of Lactobacillus supernatants. In addition, the methanol extract of Lactobacillus supernatants was fractionated using thin-layer chromatography (TLC). A solvent system consisting of methanol/chloroform is a promising approach for TLC analysis of Lactobacillus supernatant. The partial purification of the antioxidant components using thin-layer chromatography demonstrated a drastic rise in the antibacterial and antioxidant properties in comparison to the crude methanol extract of the same sample. In this study, the TLC fractionation of Lactobacillus extracellular materials was described for the first time. Further isolation and purification are essential to identify these bioactive compounds.
- Antibacterial,
- Antioxidant,
- Extraction,
- Lactobacillus,
- Supernatant,
- Thin-Layer Chromatography.
How to Cite
References
[2] Yoo JY, Kim SS. Probiotics and prebiotics: present status and future perspectives on metabolic disorders. Nutrients (2016) 8: 1–20
[3] Patrick OM. Lactic acid bacteria in health and disease. Rwanda J. Health Sci. (2012) 1: 39-50
[4] Kim H, Chae H, Jeong S, Ham J, Im S, Ahn C, Lee J. In vitro antioxidative properties of lactobacilli. Asian-Australas J. Anim Sci. (2005)19: 262.
[5] Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. (2010) 4: 118-26.
[6] Kim KT, Yang SJ, Paik HD. Probiotic properties of novel probiotic Levilactobacillus brevis KU15147 isolated from radish kimchi and its antioxidant and immune-enhancing activities. Food Sci Biotechnol. (2021) 30: 257-265.
[7] Sánchez Macarro M, Ávila-Gandía V, Pérez-Piñero S, Cánovas F, García-Muñoz AM, Abellán-Ruiz MS, Victoria-Montesinos D, Luque-Rubia AJ, et al. Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-Intensity and Duration Physical Exercise. Antioxidants (2021) 10(2): 323.
[8] Pourramezan Z, Oloomi M, Kasra Kermanshahi R, Rezadoost H. Extraction and Isolation of Antioxidant-Antibacterial Compounds From Lactobacillus casei Strain K1C by Thin-Layer Chromatography. Jundishapur J Nat Pharm Prod. (2021) 16(1): e96308.
[9] Pourramezan Z, Kasra Kermanshahi R, Oloomi M, Aliahmadi A, Rezadoost H. In vitro study of antioxidant and antibacterial activities of Lactobacillus probiotic spp. Folia Microbiol. (2018) 63(1): 31-42.
[10] Wegkamp A, Teusink B, De Vos WM, Smid EJ. Development of a minimal growth medium for Lactobacillus plantarum. Lett Appl Microbiol. (2010) 50: 57–64.
[11] Saadatzadeh A, Fazeli MR, Jamalifar H, Dinarvand R. Probiotic properties of lyophilized cell free extract of Lactobacillus casei. Jundishapur J Nat Pharm Prod. (2013) 8(3): 131–37.
[12] Choma IM, Jesionek W. TLC-direct bioautography as a high throughput method for detection of antimicrobials in plants. Chromatography (2015) 2: 225-238.
[13] Nyanzi R, Shuping DSS, Jooste PJ, Eloff JN. Antibacterial and antioxidant activity of extracts from selected probiotic bacteria. J Food Res. (2015) 4: 122
[14] Mohammadi-Motamed S, Shahidi-Motlagh S, Baghrzadeh H, Azad Forouz S, Tafazoli H. Evaluation of antioxidant activity of Ruta graveolens L. extract on inhibition of lipid peroxidation and DPPH radicals and the effects of some external factors on plant extract's potency. Res J Pharmacogn. (2014) 1: 45-50
[15] Hebraud FBADM, Sadoun D. Lactobacillus paracasei subsp. paracasei: an Algerian isolate with antibacterial activity against enteric pathogens and probiotic fitness. J Food Nutr Res. (2011) 50: 139-149.
[16] Demain AL, Fang A. The natural functions of secondary metabolites. History of modern biotechnology I (2000) 1-39.
- Abstract Viewed: 720 times
- IJPS_Volume 18_Issue 1_Pages 47-54 Downloaded: 339 times