Association of Atorvastatin with GDF9 and BMP15 Expression and in vitro Maturation of Mouse Oocytes Atorvastatin GDF9 and BMP15 Expression and Mouse Oocytes
Iranian Journal of Pharmaceutical Sciences,
Vol. 19 No. 3 (2023),
1 July 2023
,
Page 188- 193
https://doi.org/10.22037/ijps.v19i3.43843
Abstract
Atorvastatin (ATV) is one of the hyperlipidemia treatment drugs with antioxidant and anti-inflammatory effects, growth differentiation factor 9 (GDF-9) and bone morphogenetic protein 15 (BMP-15) are two potent regulator factors of ovulation. This study investigated the effect of atorvastatin on GDF-9 and BMP-15 expression and explores its relationship with oocyte maturation in mice. A total of 200 oocytes from 40 mice were collected and divided into two groups (n=100 in each group), including the control group and the atorvastatin group (receiving 2 mg/kg atorvastatin). After oocyte culture in DMEM medium, GDF-9, and BMP-15 expression were evaluated by Real-time PCR. The rate of oocyte maturation was evaluated using fluorescent microscope scanning. Data were analyzed using an ANOVA test. GDF-9 and BMP-15 expression in control and atorvastatin groups were 2.3 and 4.8 (P≤0.05) and 1.2 and 3.4 fold respectively (P≤0.001). The oocyte maturation rate in the atorvastatin and control groups was 82% and 76%, respectively (p>0.05). According to our findings, low-dose atorvastatin caused overexpression of GDF-9 and BMP-15 in mice oocytes and it could improve the quality and maturation of mouse oocytes.
- Atorvastatin
- GDF-9
- BMP-15
- Oocyte
How to Cite
References
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